recombinant gm-csf (cat Search Results


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gm csf  (R&D Systems)
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Real-Time Quantitative PCR Primer Sequence Used in the Present Study
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recombinant human gm-csf  (GENTAUR Inc)
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Real-Time Quantitative PCR Primer Sequence Used in the Present Study
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recombinant mouse gm csf  (Beijing Solarbio Science)
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Real-Time Quantitative PCR Primer Sequence Used in the Present Study
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Image Search Results


Real-Time Quantitative PCR Primer Sequence Used in the Present Study

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Neutrophil–Hepatic Stellate Cell Interactions Promote Fibrosis in Experimental Steatohepatitis

doi: 10.1016/j.jcmgh.2018.01.003

Figure Lengend Snippet: Real-Time Quantitative PCR Primer Sequence Used in the Present Study

Article Snippet: When indicated, neutralizing antibodies against GM-CSF (cat number: MAB415-100; R&D, Minneapolis, MN) and IL-15 (cat number: AF447-SP; R&D) were applied to the co-culture system.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

HSCs delay the spontaneous apoptosis of cultured neutrophils via the secretion of GM-CSF and IL-15. ( A ) Quantitative RT-PCR analyses of mRNA expression levels of cytokines and growth factors in 1-day or 5-day cultured HSCs. N = 3 in each group. ( B ) Concentrations of GM-CSF from cultured HSCs were analyzed with an enzyme-linked immunosorbent assay. Values were obtained from 4 independent experiments. ( C and D ) Quantitative RT-PCR analyses of Csf2 and Il15 mRNA expression levels from HFD+1B vs HFD groups. N = 4–6 in each group. ( E ) Csf2 and Il15 expression in primary hepatocytes and HSCs after 1-day culture. N = 3–7 in each group. ( F ) Neutrophils were cultured with 5-day cultured HSCs or HSC-conditioned medium as well as neutralizing antibodies against GM-CSF or IL-15 as indicated. The number of live cells was counted and statically analyzed. N = 4–8 in each group. * P < .05, ** P < .01, *** P < .001 vs control medium group, # P < .05 vs neutrophils + HSCs group. Egf, epidermal growth factor; Hgf, hepatocyte growth factor.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Neutrophil–Hepatic Stellate Cell Interactions Promote Fibrosis in Experimental Steatohepatitis

doi: 10.1016/j.jcmgh.2018.01.003

Figure Lengend Snippet: HSCs delay the spontaneous apoptosis of cultured neutrophils via the secretion of GM-CSF and IL-15. ( A ) Quantitative RT-PCR analyses of mRNA expression levels of cytokines and growth factors in 1-day or 5-day cultured HSCs. N = 3 in each group. ( B ) Concentrations of GM-CSF from cultured HSCs were analyzed with an enzyme-linked immunosorbent assay. Values were obtained from 4 independent experiments. ( C and D ) Quantitative RT-PCR analyses of Csf2 and Il15 mRNA expression levels from HFD+1B vs HFD groups. N = 4–6 in each group. ( E ) Csf2 and Il15 expression in primary hepatocytes and HSCs after 1-day culture. N = 3–7 in each group. ( F ) Neutrophils were cultured with 5-day cultured HSCs or HSC-conditioned medium as well as neutralizing antibodies against GM-CSF or IL-15 as indicated. The number of live cells was counted and statically analyzed. N = 4–8 in each group. * P < .05, ** P < .01, *** P < .001 vs control medium group, # P < .05 vs neutrophils + HSCs group. Egf, epidermal growth factor; Hgf, hepatocyte growth factor.

Article Snippet: When indicated, neutralizing antibodies against GM-CSF (cat number: MAB415-100; R&D, Minneapolis, MN) and IL-15 (cat number: AF447-SP; R&D) were applied to the co-culture system.

Techniques: Cell Culture, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay